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Lee Lab Research


Project Goal:To use gene expression microarrays
in the analysis of cell growth and survival

Genes induced by CSF-1

Genes repressed by CSF-1

WT: 32D myeloid cell line expressing WT-CSF-1R

DKI (dki): 32D myeloid cell line expressing DKI-CSF-1R mutant with the kinase insert deleted

p110*(p110): DKI cell line co-expressing a constitutively active PI3-kinase

Each panel shows the number of genes consistently induced or repressed following 16hrs. of CSF-1 treatment. Experiments were performed in triplicate and gene expression levels were assayed using Affymetrix microarray technology.

Current goal is to identify the genes which are induced/repressed in WT cells, not in DKI cells and restored by expression of constitutively active PI3-kinase. We are hopeful that this paradigm will allow us to identify new genes regulated by the PI3-kinase pathway in growth factor signaling.

Contact Information:
Angel W. Lee, M.D., Ph.D.