Project Goal:To use gene expression microarrays
in the analysis of cell growth and survival
Genes induced by CSF-1
Genes repressed by CSF-1
WT: 32D myeloid cell line expressing WT-CSF-1R
DKI (dki): 32D myeloid cell line expressing DKI-CSF-1R mutant with the kinase insert deleted
p110*(p110): DKI cell line co-expressing a constitutively active PI3-kinase
Each panel shows the number of genes consistently induced or repressed following 16hrs. of CSF-1 treatment. Experiments were performed in triplicate and gene expression levels were assayed using Affymetrix microarray technology.
Current goal is to identify the genes which are induced/repressed in WT cells, not in DKI cells and restored by expression of constitutively active PI3-kinase. We are hopeful that this paradigm will allow us to identify new genes regulated by the PI3-kinase pathway in growth factor signaling.